Journal: ACS pharmacology & translational science

Article Title: Cell Membrane Hybrid Liposome-Targeted Delivery of the Heat Shock Protein 90 C-Terminal Inhibitor for the Treatment of Idiopathic Pulmonary Fibrosis.

doi: 10.1021/acsptsci.4c00524

Figure Lengend Snippet: Figure 2. Silybin A has better anti-IPF efficiency than silybin B. (A) The IPF mouse model was established by BLM tracheal infusion of C57BL/6J mice, and silybin A and silybin B were administrated intragastrically 10 days later, and the mice were euthanized 14 days after administration and the lung tissue was analyzed. (B) Lung tissue morphology, H&E, and Masson staining photos of sections of mice in each group after 14 days of administration (scale = 100 μm). (C) Lung coefficient (n = 3−6). (D) Ashcroft score results of H&E-stained sections (n = 9). (E) Quantitative results of blue collagen in Masson-stained sections (n = 9) of mice in each group. (F) Contents of hydroxyproline in lung homogenate of mice (n = 3−6). (G) Contents of TGF-β1 in lung homogenate of mice (n = 3−6). (H) Contents of TNF-α in lung homogenate of mice (n = 3−6). (I) HSP90 was overexpressed in bleomycin model mouse lung sections. (J) Immunofluorescence double staining of TGFβRII (red) and HSP90 (green) of L929 cells treated with silybin A. (K) ImageJ analyzed TGFβRII and HSP90 Pearson correlation coefficients. Showing mean ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001 when compared with the control group; #P < 0.05, ##P < 0.01, and ###P < 0.001 when compared with the model group.

Article Snippet: Bleomycin hydrochloride injection (BLM) was purchased from the Takasaki plant, Nippon Kayaku Co., Ltd. Cholesterol, 1,2- didecacyl-SN-glycerol-3-phosphatidylcholine (DPPC), and 1- palmitoyl-2-oleoylphosphatidylcholine (POPC) were purchased from AVT (Shanghai) Pharmaceutical Co., Ltd. Coumarin 6 (C6) was purchased from Shanghai Yuanye BioTechnology Co., Ltd. All antibodies were purchased from Abcam (USA).

Techniques: Staining, Immunofluorescence, Double Staining, Control